mcherry激发波长是多少？
mCherry是一种来自于蘑菇珊瑚（mushroom coral）的红色荧光蛋白，常有于标记和示踪某些分子和细胞组分。相对于其他荧光，mCherry的好处在于它的颜色和应用最多的绿色荧光蛋白（GFP）能进行共同标记，并且mCherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry激发波长是多少？

mCherry是一种来自于蘑菇珊瑚（mushroom coral）的红色荧光蛋白，常有于标记和示踪某些分子和细胞组分。相对于其他荧光，mcherry的好处在于它的颜色和应用最多的GFP绿色荧光蛋白绿色荧光蛋白（GFP）能进行共同标记，并且mcherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry红色荧光蛋白
吸收和发射波长：

mcherry的更大吸收/发射峰分别位于587nm和610nm，对光致漂白耐受，荧光非常稳定。

mcherry红色荧光蛋白

常见应用：
•mCherry常用于与目的基因组成融合蛋白以及通过IRES或2A与感兴趣的蛋白共表达；
•启动子活性研究；
•荧光共振能量转移(fluorescence resonance energy transfer，FRET）和其他定量实验；
•标记细胞或者分子，进行示踪实验；

mcherry红色荧光蛋白

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 
m前缀的意义：
mCherry的m为monmer单体的缩写，表示mCherry荧光蛋白的形式为单体，这在很多实验设计中非常重要，比如与目的标记基因组成融合蛋白时。

成熟时间：
mCherry具有较快的成熟速度，t0.5为15分钟，这在一些需要做出快速反应的实验非常重要。比如启动子活性报告系统等。

如何观察和筛选mcherry在植物中的表达？

mcherry红色荧光蛋白的激发波长和发射波长

实验室常见荧光蛋白的激发波长和发射波长

紫外荧光蛋白UV Proteins

蓝色荧光蛋白Blue Proteins

青色荧光蛋白Cyan Proteins

绿色荧光蛋白Green Proteins

黄色荧光蛋白Yellow Proteins

桔色荧光蛋白Orange Proteins

红色荧光蛋白Red Proteins

远红荧光蛋白Far Red Proteins

近红荧光蛋白Near IR Proteins

Sapphire-type Proteins

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.